Fluoride as a Potential Repressor of Glycogen Metabolism in Skeletal Muscle Cell Line CCL136

The exposure of humans to fluorine is connected with its presence in the air, food and water. It well known that fluorides even at a low concentration but long time accumulate body lead numerous metabolic disorders. Fluoride recognised as factor modulating energy metabolism cells. This interaction particular importance muscle cells, which are cells high activity related glucose glycogen. In someone suffering from chronic fluoride poisoning, frequent symptoms fatigue not relieved by extra sleep or rest, muscular weakness, spasms, involuntary twitching. aim this study was examine effect concentrations determined blood people environmentally exposed on expression enzymes taking part CCL136 were cultured under standard conditions addition NaF. amount ATP produced using HPLC method, genes responsible for glycogen WB RT PCR methods fluorimetric PAS methods. has been shown 1, 3 10 μM NaF there change state pattern involved synthesis breakdown observed caused decrease content also increased deposition. These changes accompanied gene level enzymatic proteins metabolism: synthase, synthase kinase phosphorylase. results obtained shed new light molecular mechanisms acts an environmental toxin.